The expression of B-50/GAP-43 in Schwann cells is upregulated in degenerating peripheral nerve stumps following nerve injury
Identifieur interne : 004606 ( Main/Exploration ); précédent : 004605; suivant : 004607The expression of B-50/GAP-43 in Schwann cells is upregulated in degenerating peripheral nerve stumps following nerve injury
Auteurs : L. C. Plantinga [Pays-Bas] ; J. Verhaagen [Pays-Bas] ; P. M. Edwards [Royaume-Uni] ; E. M. Hol [Pays-Bas] ; P. R. B R [Pays-Bas] ; W. H. Gispen [Pays-Bas]Source :
- Brain Research [ 0006-8993 ] ; 1993.
English descriptors
- KwdEn :
- Teeft :
- Agarose, Axon, Axonal, Axonal regeneration, Biol, Cdna, Cdna probe, Cell contact, Control nerves, Control rats, Crush, Crush injury, Distal nerve portion, Distal nerve stump, Distal portion, Dorsal, Dorsal root ganglia, Ethidium bromide, Fibroblast, Ganglion, Gene expression, Gispen, Growth cones, Hybridization, Hybridized, Immunoreactivity, Intact sciatic nerve, Lower panel, Mrna, Mrna expression, Nerve, Nerve crush, Nerve injury, Nerve transection, Neuron, Neuronal, Neurosci, Northern blot analysis, Oestreicher, Peritraumatic, Polymerase, Polymerase chain reaction, Primer, Protein kinase, Proximal, Proximal portion, Reactive schwann cells, Regeneration, Rinsed, Schwann, Schwann cells, Sciatic, Sciatic nerve, Sciatic nerve crush, Sciatic nerves, Spinal cord, Stump, Transected, Transected sciatic nerves, Transection, Upper panel, Verhaagen.
Abstract
Abstract: We have detected mRNA for B-50 (GAP-43, pp46, F1, neuromodulin), which was originally believed to be a neuron-specific protein, in non-neuronal cells in the rat sciatic nerve. In control rats, the level of B-50 mRNA in sciatic nerve tissue was much lower than in dorsal root ganglia. Following nerve crush or transection, the expression of B-50 mRNA in the distal nerve stump increased dramatically between 1 and 2 days post-injury. The B-50 mRNA levels in the distal stump of crushed nerves remained elevated for up tp 4 weeks and subsequently returned to control levels after 7 weeks. In contrast, after nerve transection B-50 mRNA levels in the distal nerve portion continued to increase up to 7 weeks post-lesion. No changes in the levels of the B-50 transcript were observed in the proximal portion of either crush-lesioned or transected sciatic nerves. In situ hybridization demonstrated B-50 mRNA associated with Schwann cells in the distal nerve stump. The observation that Schwann cells are capable of producing B-50 mRNA was confirmed by Northern blot analysis of total RNA isolated from primary Schwann cell cultures. Taken together, these data show the expression of B-50 mRNA by Schwann cells and the up-regulation of B-50 mRNA in reactive Schwann cells upon loss of axonal contact.
Url:
DOI: 10.1016/0006-8993(93)90243-G
Affiliations:
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C." last="Plantinga">L. C. Plantinga</name><affiliation wicri:level="4"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Rudolf Magnus Institute, Department of Pharmacology, Utrecht University</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Verhaagen, J" sort="Verhaagen, J" uniqKey="Verhaagen J" first="J." last="Verhaagen">J. Verhaagen</name><affiliation></affiliation><affiliation wicri:level="4"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Rudolf Magnus Institute, Department of Pharmacology, Utrecht University</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Edwards, P M" sort="Edwards, P M" uniqKey="Edwards P" first="P. M." last="Edwards">P. M. Edwards</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Molecular Genetics Unit, Bacteriology Department, Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, New Haw, Weybridge</wicri:regionArea><wicri:noRegion>Weybridge</wicri:noRegion></affiliation></author><author><name sortKey="Hol, E M" sort="Hol, E M" uniqKey="Hol E" first="E. M." last="Hol">E. M. Hol</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Neurology, University of Utrecht</wicri:regionArea><wicri:noRegion>University of Utrecht</wicri:noRegion></affiliation></author><author><name sortKey="B R, P R" sort="B R, P R" uniqKey="B R P" first="P. R." last="B R">P. R. B R</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Neurology, University of Utrecht</wicri:regionArea><wicri:noRegion>University of Utrecht</wicri:noRegion></affiliation></author><author><name sortKey="Gispen, W H" sort="Gispen, W H" uniqKey="Gispen W" first="W. H." last="Gispen">W. H. Gispen</name><affiliation wicri:level="4"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Rudolf Magnus Institute, Department of Pharmacology, Utrecht University</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author></analytic><monogr></monogr><series><title level="j">Brain Research</title><title level="j" type="abbrev">BRES</title><idno type="ISSN">0006-8993</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">602</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="69">69</biblScope><biblScope unit="page" to="76">76</biblScope></imprint><idno type="ISSN">0006-8993</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-8993</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Axotomy</term><term>B-50</term><term>GAP-43</term><term>Peripheral nerve regeneration</term><term>Schwann cell</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Agarose</term><term>Axon</term><term>Axonal</term><term>Axonal regeneration</term><term>Biol</term><term>Cdna</term><term>Cdna probe</term><term>Cell contact</term><term>Control nerves</term><term>Control rats</term><term>Crush</term><term>Crush injury</term><term>Distal nerve portion</term><term>Distal nerve stump</term><term>Distal portion</term><term>Dorsal</term><term>Dorsal root ganglia</term><term>Ethidium bromide</term><term>Fibroblast</term><term>Ganglion</term><term>Gene expression</term><term>Gispen</term><term>Growth cones</term><term>Hybridization</term><term>Hybridized</term><term>Immunoreactivity</term><term>Intact sciatic nerve</term><term>Lower panel</term><term>Mrna</term><term>Mrna expression</term><term>Nerve</term><term>Nerve crush</term><term>Nerve injury</term><term>Nerve transection</term><term>Neuron</term><term>Neuronal</term><term>Neurosci</term><term>Northern blot analysis</term><term>Oestreicher</term><term>Peritraumatic</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Primer</term><term>Protein kinase</term><term>Proximal</term><term>Proximal portion</term><term>Reactive schwann cells</term><term>Regeneration</term><term>Rinsed</term><term>Schwann</term><term>Schwann cells</term><term>Sciatic</term><term>Sciatic nerve</term><term>Sciatic nerve crush</term><term>Sciatic nerves</term><term>Spinal cord</term><term>Stump</term><term>Transected</term><term>Transected sciatic nerves</term><term>Transection</term><term>Upper panel</term><term>Verhaagen</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We have detected mRNA for B-50 (GAP-43, pp46, F1, neuromodulin), which was originally believed to be a neuron-specific protein, in non-neuronal cells in the rat sciatic nerve. In control rats, the level of B-50 mRNA in sciatic nerve tissue was much lower than in dorsal root ganglia. Following nerve crush or transection, the expression of B-50 mRNA in the distal nerve stump increased dramatically between 1 and 2 days post-injury. The B-50 mRNA levels in the distal stump of crushed nerves remained elevated for up tp 4 weeks and subsequently returned to control levels after 7 weeks. In contrast, after nerve transection B-50 mRNA levels in the distal nerve portion continued to increase up to 7 weeks post-lesion. No changes in the levels of the B-50 transcript were observed in the proximal portion of either crush-lesioned or transected sciatic nerves. In situ hybridization demonstrated B-50 mRNA associated with Schwann cells in the distal nerve stump. The observation that Schwann cells are capable of producing B-50 mRNA was confirmed by Northern blot analysis of total RNA isolated from primary Schwann cell cultures. Taken together, these data show the expression of B-50 mRNA by Schwann cells and the up-regulation of B-50 mRNA in reactive Schwann cells upon loss of axonal contact.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li><li>Royaume-Uni</li></country><region><li>Utrecht (province)</li></region><settlement><li>Utrecht</li></settlement><orgName><li>Université d'Utrecht</li></orgName></list><tree><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="Plantinga, L C" sort="Plantinga, L C" uniqKey="Plantinga L" first="L. C." last="Plantinga">L. C. Plantinga</name></region><name sortKey="B R, P R" sort="B R, P R" uniqKey="B R P" first="P. R." last="B R">P. R. B R</name><name sortKey="Gispen, W H" sort="Gispen, W H" uniqKey="Gispen W" first="W. H." last="Gispen">W. H. Gispen</name><name sortKey="Hol, E M" sort="Hol, E M" uniqKey="Hol E" first="E. M." last="Hol">E. M. Hol</name><name sortKey="Verhaagen, J" sort="Verhaagen, J" uniqKey="Verhaagen J" first="J." last="Verhaagen">J. Verhaagen</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Edwards, P M" sort="Edwards, P M" uniqKey="Edwards P" first="P. M." last="Edwards">P. M. Edwards</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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